Acute lymphoblastic leukemia typing

1. Typing 1986 Tianjin held leukemia classification type seminars, will be divided into seven-ANLL, diagnostic criteria are as follows:
(1) acute myeloid leukemia undifferentiated (M1) Zhongyuan tablets of bone marrow cells (I + II type) ≥ 90% (non-erythroid cells), promyelocytic cell small, neutral in the promyelocytic cell following stage but not rare or .
(2) acute myeloid leukemia part differentiated (M2) in two subtypes, ① M2a: Zhongyuan tablets of bone marrow cells (type I +)> 30 to <90%> 10%; ② M2b: primitive bone marrow abnormalities in the early promyelocytic cells markedly increased, in a neutral abnormal cell proliferation David tablets mainly its nucleus often nucleolus, with a clear nuclear Pulp uneven development of such cells> 30%.
(3) Particle number of acute promyelocytic leukemia (M3) in the bone marrow to increase the particle abnormal promyelocytic cell hyperplasia mainly> 30% (non-erythroid cells), the size of its nucleus, and in the cytoplasm a range of particle sizes. There are two subtypes, ① coarse particle type (M3a):toluidine blue coarse particles, intensive or fusion; ② fine particle type (M3b):toluidine blue-intensive and small particles.
(4) acute tablets - monocytic leukemia (M4) in accordance with the original tablets and monocyte cell line patterns different, which may include the following four subtypes: ① M4a: primitive and promyelocytic cell hyperplasia mainly, the original single and David monocytes cells> 20% (non-erythroid cells); ② M4b: The original, David mononuclear cell proliferation mainly original and promyelocytic cells> 20% (non-erythroid cells); ③ M4c: primitive cells of both tablets, a mononuclear cell line characteristics are> 30%; ④ M4ED: In addition to the above features, there are large and round, eosinophil granules coloring deeper eosinophil, accounting for 5 to 30 percent.
(5) acute monocytic leukemia (M5) in two subtypes: ① undifferentiated (M5a): primitive bone marrow mononuclear cells (type I + II) (non-erythroid cells) ≥ 80%; ② some differentiation type (M5b): Bone marrow cells in the primitive and naive> 30% (non-erythroid cells); original mononuclear cells (I + II type) <80%.
(6) K562 (M6) red blood cells in the bone marrow of> 50%, and often abnormal morphology of the original tablets cells (type I + II), (or the original 10 David mononucleosis)> 30%; blood tablets tablets of the Central Plains (I + II type) (or the original single) cells> 5%, non-bone marrow cells Zhongyuan granulocyte erythroid cells (primitive or 10 David mononucleosis)> 20%.
(7) megakaryocytic leukemia (M7) is the original blood megakaryocyte (small megakaryocyte) cells; Zhongyuan bone marrow megakaryocyte cells> 30%. The original megakaryocyte cells or groups of monoclonal antibodies electron microscopy confirmed; bone marrow hematopoietic cells was less often "dry pumping", the original biopsy and a megakaryocyte cells increased, mesh fiber increased.